Computer softwares

Huygens deconvolution for confocal and widefield microscopy:



Free online calculators from Scientific Volume Imaging:


Computer softwares developed at the Department of Biophysics and Cell Biology:

AccPbFRET Acceptor Photobleaching FRET evaluation for microscopy

Intensity-based Ratiometric FRET evaluation for microscopy

FRET evaluation for flow cytometry

Excel spreadsheet for calculating FRET efficiency from ensemble data (medians or trimmed means of cell or pixel populations), with pre-loaded spectral data for the most commong FRET fluorophores

Updated: 2021.12.20.

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